Cell Metric® CLD

Cell Metric® CLD is our premier, bench-top system for cell line development.

Groups that benefit from this system typically have throughput requirements from tens to hundreds of plates. The Cell Metric® CLD includes an integrated, temperature-controlled plate stacker. A batch of up to 10 lidded plates is loaded manually into the stacker and the plates are automatically transferred for imaging one at a time with barcode reading.Cell Metric CLD

Just like the Cell Metric®, the Cell Metric® CLD uses non-invasive brightfield imaging for analysis of settled suspension or adherent cells. Cells are imaged in lidded, flat-bottomed microplates (384, 96, 24, 6-well) in their normal liquid media environment.

The software is quick to set up with the same parameters used across the whole batch, and a full plate can be scanned from under 3 minutes, with whole-well images captured at single-cell resolution. Results are reviewed on the instrument computer or at your desk using the remote viewer option.

The software recognises the plate and well ID and automatically plots growth curves over a time course. The software also highlights wells of interest based on your defined protocols and stores photo-evidence of monoclonality. Tracking of wells together with the associated images and data allows you to manage your workflow.

The Cell Metric® CLD platform is an extremely cost effective alternative to third party integrated robotic systems comprised of an incubator, robot arm and scheduling software.

  • It is designed specifically for cell line development with emphasis on the clone screening element of the workflow.
  • It is ideal for groups that want to expand their cell line development capacity.
  • It is ideal for customers who want to replace their first-generation clone-screening imagers.

The Cell Metric® CLD can now be purchased with fluorescence detection features that enable the user to validate a new a seeding method or quality check their existing method. The factory fitted feature provides the user with the option to use red or green fluorescence channels, together or separately, for the method qualification and the outputs generated allow for either quick review of their seeding performance by the user or provide statistics available for export and further details data analysis.

To learn how Cell Metric® CLD can accelerate your workflow, see Cell line development.
For lower-throughput requirements, see Cell Metric®.
For highest-throughput requirements, see Cell Metric® rapid.

Key metrics

Metric measuredDescriptionGraphic representation
Single cell confirmation Single cell resolution image of whole well on day 0 Heat map of clonal cells. Photo-documentation at day 0
Confluence Measures percentage coverage or carpeting of the well base Displays % confluence for each well
Growth rates Uses the cell count to generate and automatically update (using plate and well ID) growth curves for each clone Growth curve plots – used to look at stability and performance in different growth media and conditions
Two channel fluorescence – Red and Green Fluorescing loci (cells) can be quickly detected in an image of a whole well on day 0 and be used to aid verification of the cloning method Plate overview map of wells with the fluorescent object count (both colours)

 

Features

FeatureBenefits
Highest-quality and highest-resolution images Enables single-cell resolution
Measures cells at day 0 straight after dispensing into plates.
Avoids the need for subcloning of colonies and potentially shaves weeks off the cell line development process
Cells are detected in microplate wells

Accurate cell identification.
Direct visual read with no pipetting errors from the well or perturbation of the cells.
Unlimited number of data points

Non-invasive: no stains, labels or dyes No sampling required and can make an unlimited number of consistent measurements of whole-well cell number. Growth curves generated for single wells.
Important to avoid fluorescent labels where cell lines are intended for clinical and therapeutic candidates
Incubated plate stacker for batch of 10 plates Cells remain in consistent, favourable conditions.
Increased walkaway time (around 45 mins per batch) frees up lab personnel for other tasks
Read times from under 3 minutes per plate Higher speed and accuracy compared to manual microscope method.
Highest chance that cell has not divided by the time a well is read
Automatic focusing across the well and across the plate Consistency. No wells are lost through being out of focus
Even illumination and whole well read up to edges High image quality with no shadowing or halos at the well edges. This performance is needed for clone screening as single cells are often located at the edge of the well
Works with all SBS-style plate formats Flexible applications from transfection pools through to candidate selection
Digital record of cells Regulatory proof and audit trail
Windows software package Simple imaging set up, results analysis and data export
Optional 2 channel fluorescence detection (Red and Green) Allows for verification or QC of cloning method using fluorescently labelled cells

 

Physical specifications

Dimensions (H x W x D)
650 mm x 465 mm x 500 mm
25.6” x 18.3" x 19.7"

(Instrument only, excludes PC and monitor)

Weight
50 kg, 110 lbs

Computer
Dell Precision™ workstation
Intel® Xeon® processor
500 GB hard disk
Microsoft Windows 7, 64-bit
24" flat screen monitor

Image acquisition
Illumination: brightfield
Resolution: 2 µm per pixel
Image format: images stored in TIFF format
Acquisition speed: for a 96-well microplate, from <3 minutes for full scan

Cell culture containers
Most cell culture vessels that fit in an SBS microplate format footprint (128 x 86 mm) can be imaged, including 6, 12, 24, 48, 96, 384 and 1536-well plates. High-quality plates produce the best-quality images.
For more details and for other cell culture vessel formats, contact us.

Integrated plate loader
Holds 10 lidded SBS-format microtitre plates
Adjustable temperature control (supports 37°C)
Built-in barcode reader

For research use only.


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