Cells containing the transgene are typically seeded using one of the following three approaches:
With any of these methods, including the single-cell cloning methods, there remains a degree of uncertainty as to whether the resulting colony is monoclonal (derived from a single cell). However, by employing the Cell Metric CLD™ at this early stage for both limiting dilutions and FACS-based approaches, rapid screening of the entire population can be achieved immediately after seeding while still at the single cell level. In this way the Cell Metric CLD™ fully documents the evidence for single-cell deposition and hence monoclonality (see Clone screening).
Seeded microplates are taken forwards for clone screening to identify monoclonal populations.
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