Single cell cloning

The requirements are to dispense single cells from a pooled population into wells of 96 well microtiter plates.

This should be done at high efficiency so that most wells contain a single cell and they need to be viable so they grow into a colony over the next few days.

FACS and Cell Printers can damage adherent cells leading to low viabilities, and hence many customers still wish to use a gentler limiting dilutions approach.

Due to low transfection efficiencies, for gene knock-outs single cell cloning will typically require a few hundred clones to be plated. For knock-ins, these occur at much lower frequencies, and may need over a 1000 clones per edit.

Solentim have now developed a system called the VIPS, based on a SMART™ limiting dilutions approach, as the method of choice for single cell cloning.

The VIPS will generate a resultant plate with high cloning efficiency (see plate image map below) in around 10 minutes and due to the gentle nature of the dispensing method, most of these wells will go on and grow up successfully to form colonies.

VIPS System

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